Adding a Cytoband

Click the + Add reference files button under the Reference Files section header and choose Cytoband from the Library type drop-down list in the Add reference sequence dialog (Figure 1, left). If a reference sequence is already associated with an assembly, Cytoband will be the only option available in the Add reference sequence dialog and will not appear in a drop-down list (Figure 1, right).

Figure 1. Add reference sequence dialog. If the reference sequence and cytoband files have not been added yet, both options will appear in the Library type drop-down list (left). If one is missing, it will appear as the only option (right). For many model organisms, automatic downloads are available from the Connected Multiomics repository.

If you are using an assembly supported by Connected Multiomics (e.g. human), select Download cytoband and click Create to get the cytoband file from the Connected Multiomics repository. Alternatively, select Build cytoband and select .fasta files or .2bit files to to build the cytoband file. If the reference sequence is missing, it will either be downloaded automatically or you will be asked to import it from another source.

If you are using a custom assembly (e.g. for a non-model organism), the Download cytoband option will not be available (Figure 2).

Figure 2. For custom assemblies, automatic downloads are not available so the cytoband has to be created.