> For the complete documentation index, see [llms.txt](https://help.multiomics.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.multiomics.illumina.com/icm/reference/list-of-analysis-tasks-by-data-type/spatial-transcriptomics.md).

# Spatial Transcriptomics

## Expression Count Matrix

Examples of expression count matrix are Spatial - Cells, Single cell counts, Filtered counts, Normalized counts. Analysis tasks that can be invoked from expression count matrix are listed below.

| Task Menu Section         | Analysis Task Name                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      |
| ------------------------- | ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- |
| Report                    | <ul><li>Spatial report</li></ul>                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        |
| Annotation/Metadata       | <ul><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/annotation-metadata/annotate-cells">Annotate cells</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/annotation-metadata/annotate-cells-1">Annotate features</a></li><li>Annotate Visium image</li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/annotation-metadata/publish-cell-attributes-to-project">Publish attributes to study</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/annotation-metadata/annotate-cells-2">Attribute report</a></li></ul>                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           |
| QA/QC                     | <ul><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/qa-qc/feature-distribution">Feature distribution</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/qa-qc/single-cell-qa-qc">Single cell QA/QC</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/qa-qc/cell-barcode-qa-qc">Cell barcode QA/QC</a></li></ul>                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              |
| Pre-analysis tools        | <ul><li>Hashtag demultiplexing (Available on normalized data only)</li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/pre-analysis-tools/pool-cells">Pseudobulk</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/pre-analysis-tools/split-matrix">Split by feature type</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/pre-analysis-tools/generate-group-cell-counts">Generate group cell counts</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/pre-analysis-tools/merge-matrices">Merge matrices</a></li></ul>                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               |
| Filtering                 | <ul><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/filtering/filter-features">Filter features</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/filtering/filter-groups-samples-or-cells">Filter cells</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/filtering/split-by-attribute">Split by attribute</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/filtering/downsample-cells">Downsample cells</a></li></ul>                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               |
| Normalization and scaling | <ul><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/normalization-and-scaling/normalization">Normalization</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/normalization-and-scaling/normalize-to-baseline">Normalization to baseline</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/normalization-and-scaling/scran-deconvolution">Scran deconvolution</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/normalization-and-scaling/tf-idf-normalization">TF-IDF normalization</a></li><li>Scale expression (Available on normalized data only)</li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/normalization-and-scaling/impute-missing-values">Impute missing values</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/normalization-and-scaling/sctransform">SCTransform</a></li></ul>                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              |
| Batch removal             | <ul><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/batch-removal/general-linear-model">General linear model</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/batch-removal/seurat3-integration">Seurat3 integration</a> (Available on normalized data only)</li></ul>                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           |
| Statistics                | <ul><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/statistics/differential-analysis">Differential analysis</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/statistics/correlation-analysis-1">Correlation</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/statistics/correlation-analysis">Descriptive statistics</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/statistics/compute-biomarkers">Compute biomarkers</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/statistics/survival-analysis-with-cox-regression-and-kaplan-meier-analysis#cox-regression">Cox Regression</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/statistics/survival-analysis-with-cox-regression-and-kaplan-meier-analysis#kaplan-meier-survival-curve">Kaplan-Meier</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/statistics/spatially-variable-genes">Spatially Variable Genes</a> (Available on non-normalized data only)</li></ul>                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 |
| Exploratory analysis      | <ul><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/exploratory-analysis/graph-based-clustering">Graph-based clustering</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/exploratory-analysis/k-means-clustering">K-means clustering</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/exploratory-analysis/compare-clusters">Compare clusters</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/exploratory-analysis/pca">PCA</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/exploratory-analysis/t-sne">t-SNE</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/exploratory-analysis/umap">UMAP</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/exploratory-analysis/hierarchical-clustering">Hierarchical clustering / heatmap</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/exploratory-analysis/aucell">AUCell</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/exploratory-analysis/svd">SVD</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/exploratory-analysis/banksy-spatial-domain-identification">BANSKY - Spatial Domain Identification</a> (Available on non-normalized data only)</li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/exploratory-analysis/cellphonedb">CellPhoneDB</a> (Available on normalized data only)</li></ul> |
| Trajectory analysis       | <ul><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/trajectory-analysis/trajectory-analysis-monocle-2">Monocle 2</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/trajectory-analysis/trajectory-analysis-monocle-3">Monocle 3</a></li></ul>                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     |
| Biological interpretation | <ul><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/biological-interpretation/gsea">GSEA</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/biological-interpretation/gsea-1">Gene set ANOVA</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/biological-interpretation/gene-set-enrichment">Gene set enrichment</a></li></ul>                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              |
| Classification            | <ul><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/classification/classify-cell-type">Classify cell type</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/classification/sctype">ScType cell classification</a> (Available on non-normalized data only)</li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/classification/train-classifier">Train classifier</a></li></ul>                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     |
| Conversion                | <ul><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/conversion">Export to h5ad files</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/conversion">Export matrix</a></li></ul>                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    |

## PCA

Analysis tasks that can be invoked from PCA data node are listed below.

| Task Menu Section    | Analysis Task Name                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  |
| -------------------- | ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- |
| Annotation/Metadata  | <ul><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/annotation-metadata/publish-cell-attributes-to-project">Publish attributes to study</a></li></ul>                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               |
| Batch removal        | <ul><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/batch-removal/general-linear-model">General linear model</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/batch-removal/harmony">Harmony</a></li></ul>                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   |
| Statistics           | <ul><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/statistics/differential-analysis">Differential analysis</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/statistics/correlation-analysis-1">Correlation</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/statistics/correlation-analysis">Descriptive statistics</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/statistics/compute-biomarkers">Compute biomarkers</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/statistics/survival-analysis-with-cox-regression-and-kaplan-meier-analysis#cox-regression">Cox Regression</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/statistics/survival-analysis-with-cox-regression-and-kaplan-meier-analysis#kaplan-meier-survival-curve">Kaplan-Meier</a></li></ul>                           |
| Exploratory analysis | <ul><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/exploratory-analysis/graph-based-clustering">Graph-based clustering</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/exploratory-analysis/k-means-clustering">K-means clustering</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/exploratory-analysis/compare-clusters">Compare clusters</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/exploratory-analysis/t-sne">t-SNE</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/exploratory-analysis/umap">UMAP</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/exploratory-analysis/find-multimodal-neighbors">Find multimodal neighbors</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/exploratory-analysis/svd">SVD</a></li></ul> |
| Conversion           | <ul><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/conversion">Export matrix</a></li></ul>                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         |

## Differential Analysis Result

Analysis tasks that can be invoked from differential analysis result (e.g., ANOVA, Wilcoxon) are listed below.

| Task Menu Section         | Analysis Task Name                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       |
| ------------------------- | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ |
| QA/QC                     | <ul><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/qa-qc/feature-distribution">Feature distribution</a></li></ul>                                                                                                                                                                                                                                                                                                                                                                                                       |
| Filtering                 | <ul><li>Differential analysis filter</li></ul>                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           |
| Statistics                | <ul><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/statistics/correlation-analysis">Descriptive statistics</a></li></ul>                                                                                                                                                                                                                                                                                                                                                                                                |
| Exploratory analysis      | <ul><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/exploratory-analysis/hierarchical-clustering">Hierarchical clustering / heatmap</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/exploratory-analysis/merge-differential-expression-results">Merge differential expression results</a></li></ul>                                                                                                                                                              |
| Motif detection           | <ul><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/motif-detection#detect-de-novo-motifs">Detect de novo motifs</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/motif-detection#search-for-known-motifs">Search for known motifs</a></li></ul>                                                                                                                                                                                                                  |
| Biological interpretation | <ul><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/biological-interpretation/gene-set-enrichment">Gene set enrichment</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/biological-interpretation/correlation-engine-atlases">Correlation Engine atlases</a></li><li><a href="https://help.multiomics.illumina.com/icm/analyses/analysis-functionality/task-menu/biological-interpretation/correlation-engine-pathways">Correlation Engine pathways</a></li></ul> |


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