Get targeted mRNA

On a data node contains miRNA list, e.g. differential analysis result data, miRNA integration > Get targeted mRNA task will be enabled.

Database like TargetScan can be used to get targeted mRNAs for a specific mRNA. Specify the database in the dialog, and click Finish.

Double click on the Targeted mRNA node to open the report

• Context++ score: estimating the strength of repression, more negative values suggest stronger predicted repression, this is the key metric for ranking predicted targets

• Context++ score percentile: percentile ranking of the score compared to all predictions, higher percentile means stronger predicted effect

• Weighted Context++ score: adjusted score considering multiple sites in the same transcript

• Predicted relative KD: predicted relative dissociation constant is to measure the affinity between miRNA and targeted mRNA, lower KD means stronger binding affinity, higher KD means weaker binding.