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  1. Run Set Up

Sample Sheet Introduction

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Last updated 3 months ago

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A sample sheet is required for each analysis with DRAGEN Single Cell RNA software. A sample sheet is a comma-separated value (*.csv) file format used by Illumina instruments, platforms, and analysis pipelines to store settings and data for sequencing and analysis. The DRAGEN Single Cell RNA software is compatible with the sample sheet v2. For general information on the sample sheet v2, refer to .

The sample sheet includes a list of samples and their index sequences, along with additional information required to run DRAGEN Single Cell RNA software. Appropriate index adapter sequences are determined by the assay used to perform analysis.

When running analysis on ICA, a valid sample sheet can be created by:

  • BaseSpace Run Planner (preferred), see for details

  • Downloading and modifying a sample sheet template following the requirements, see for details

Illumina Connected Software - Sample Sheet
Run Planning in BaseSpace Sequence Hub
Sample Sheet Requirements page