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  • Introduction
  • Get Started
    • Prerequisites
  • Run Set Up
    • Sample Sheet Introduction
    • Run Planning in BaseSpace Sequence Hub
    • Sample Sheet Requirements
    • Manual Launch on BaseSpace
  • Analysis Methods
    • FASTQ Processing
    • Error Correction
    • Transcript Counting
  • Analysis Results
    • Accessing Results
    • DRAGEN Report
      • Single-Cell RNA
      • Trimmer
      • DRAGEN FastQC
      • Mapping
    • Secondary Analysis Results
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  1. Get Started

Prerequisites

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Last updated 3 months ago

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  • NovaSeq 6000/6000Dx, NextSeq 2000, or NovaSeq X Series

  • Illumina Single Cell 3' RNA Prep Kit

    • T2: 1 library per sample, 1 FASTQ pair

    • T10: 1 library per sample, 1 FASTQ pair

    • T20: 1 library per sample, 1 FASTQ pair

    • T100: 4 libraries per sample, 1 FASTQ pair

    • T1000: 8 libraries per sample, 8 FASTQ pairs

    • For more information about the library preparation kit, refer to the .

  • A cloud account with a valid subscription. For information on registering your BaseSpace Sequence Hub or Illumina Connected Analytics account, refer to .

Illumina Single Cell Prep Support Site
Software Registration page