Known Issues and Limitations

DRAGEN miRNA 1.0.0

Known Issues​

• Sample-level metrics: DRAGEN Reports currently do not display sequencing quality graphs at the sample level.

• UMI support: DRAGEN Reports currently display metrics for Reads, but not for Unique Molecular Identifiers (UMIs).​ Please refer to the UMI output files (e.g., all_samples.miRNA.UMIs.txt) for UMI-specific information.​

• Summary file counts: In the files all_samples.V1.summary.xlsx and all_samples.V1.summary.txt, the reported total reads​ for each small RNA type (e.g., miRNA_Reads, hairpin_Reads, piRNA_Reads, rRNA_Reads, tRNA_Reads, mRNA_Reads) represent​ the sum of all reads mapped to that RNA category. However, only small RNAs with at least 10 read matches are listed in​ the individual small RNA output files.•

Known Limitations​ ​

• RNA detection is restricted to the reference sequences provided by the software for the supported model organisms.​ Custom or user-supplied references are not supported.​

• Only RNAs included in the reference database are detected; the software does not support discovery or prediction of​ novel or unannotated RNAs.