# Known Issues and Limitations

## DRAGEN miRNA 1.0.0

### Known Issues​<br>

* Sample-level metrics: DRAGEN Reports currently do not display sequencing quality graphs at the sample level.
* UMI support: DRAGEN Reports currently display metrics for Reads, but not for Unique Molecular Identifiers (UMIs).​ Please refer to the UMI output files (e.g., all\_samples.miRNA.UMIs.txt) for UMI-specific information.​
* Summary file counts: In the files all\_samples.V1.summary.xlsx and all\_samples.V1.summary.txt, the reported total reads​ for each small RNA type (e.g., miRNA\_Reads, hairpin\_Reads, piRNA\_Reads, rRNA\_Reads, tRNA\_Reads, mRNA\_Reads) represent​ the sum of all reads mapped to that RNA category. However, only small RNAs with at least 10 read matches are listed in​ the individual small RNA output files.•

### Known Limitations​ ​

* RNA detection is restricted to the reference sequences provided by the software for the supported model organisms.​ Custom or user-supplied references are not supported.​
* Only RNAs included in the reference database are detected; the software does not support discovery or prediction of​ novel or unannotated RNAs.


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